凈化工(gong)(gong)作(zuo)(zuo)(zuo)臺(tai)是一(yi)(yi)種局(ju)部層流(liu)(liu)裝置,能在局(ju)部形成(cheng)高潔度的(de)(de)工(gong)(gong)作(zuo)(zuo)(zuo)環境(jing)。它(ta)由(you)工(gong)(gong)作(zuo)(zuo)(zuo)臺(tai)、過(guo)(guo)濾(lv)(lv)器(qi)(qi)、風機、靜壓箱和(he)支撐體等組成(cheng),采用過(guo)(guo)濾(lv)(lv)空氣(qi)使(shi)工(gong)(gong)作(zuo)(zuo)(zuo)臺(tai)操作(zuo)(zuo)(zuo)區(qu)達到凈化除(chu)菌(jun)的(de)(de)目的(de)(de)。室內空氣(qi)經(jing)預過(guo)(guo)濾(lv)(lv)器(qi)(qi)和(he)高效過(guo)(guo)濾(lv)(lv)除(chu)塵(chen)后以垂直(zhi)或水平層流(liu)(liu)狀(zhuang)(zhuang)態(tai)通過(guo)(guo)工(gong)(gong)作(zuo)(zuo)(zuo)臺(tai)的(de)(de)操作(zuo)(zuo)(zuo)區(qu),由(you)于空氣(qi)沒有渦流(liu)(liu),所以,任何一(yi)(yi)點灰塵(chen)或附著在灰塵(chen)上的(de)(de)雜菌(jun)都能被排除(chu),不易向別處擴散和(he)轉(zhuan)移。因此,可使(shi)操作(zuo)(zuo)(zuo)區(qu)保持無菌(jun)狀(zhuang)(zhuang)態(tai)。
與無茵操作室室和預防接種箱會比較,安全實用靜化運轉臺體現了運轉必要條件好、基本操作非常方便、無茵操作室郊果可靠的、無進行消毒化學劑對人為害、占用量體積小且可運動等優缺。假如放到無茵操作室窒內安全實用,無茵操作室郊果最好。其優點缺點是價值高端,預吸附器和科學規范吸附器還還要死期拆洗和更換。 高壓低壓水蒸汽無菌鍋高壓(ya)蒸(zheng)汽滅菌(jun)鍋(guo)(guo)是一個密(mi)閉(bi)的、可(ke)以耐受一定壓(ya)力(li)(li)的雙層(ceng)金屬鍋(guo)(guo)。鍋(guo)(guo)底或(huo)夾(jia)層(ceng)內盛水,當水在鍋(guo)(guo)內沸騰(teng)時(shi)由于蒸(zheng)汽不能逸出,使鍋(guo)(guo)內壓(ya)力(li)(li)逐漸升(sheng)高,水的沸點和溫(wen)度可(ke)隨(sui)之升(sheng)高,從而達(da)到高溫(wen)滅菌(jun)的目的。一般在0.11MPa的壓(ya)力(li)(li)下,121℃滅菌(jun)20~30min,包括芽孢在內的所有(you)微(wei)生物(wu)均可(ke)被殺死。如果滅菌(jun)物(wu)品體積較大,蒸(zheng)汽穿透(tou)困(kun)難,可(ke)以適當提高蒸(zheng)汽壓(ya)力(li)(li)或(huo)延長滅菌(jun)時(shi)間。
油田殺菌鍋有臥式、雙面、臺式式等幾類型、,在分子生物學學學檢測室,非常經常用的是臺式式和雙面油田蒸汽加熱殺菌鍋。和臥式儲罐殺菌鍋相對比,油田殺菌鍋的長處是殺菌所需要的時間間隔短、節電清潔燃料、殺菌完全等。其弱項是定價過高,殺菌數量較小。 塑造箱培(pei)(pei)養(yang)(yang)箱(xiang)(xiang)(xiang)(xiang)是培(pei)(pei)養(yang)(yang)微(wei)(wei)生(sheng)物(wu)(wu)的(de)(de)專(zhuan)用(yong)(yong)(yong)設(she)備(bei)。制熱式培(pei)(pei)養(yang)(yang)箱(xiang)(xiang)(xiang)(xiang)是由電(dian)爐絲和溫度(du)(du)控(kong)制儀合成的(de)(de)固定體(ti)積的(de)(de)恒(heng)(heng)溫培(pei)(pei)養(yang)(yang)裝置,大(da)小規格(ge)不一。微(wei)(wei)生(sheng)物(wu)(wu)實驗室常用(yong)(yong)(yong)的(de)(de)培(pei)(pei)養(yang)(yang)箱(xiang)(xiang)(xiang)(xiang)工作容積有(you)(you)450×450×350mm3或(huo)650×500×500mm3,適(shi)用(yong)(yong)(yong)于(yu)室溫至60℃之(zhi)間的(de)(de)各(ge)類微(wei)(wei)生(sheng)物(wu)(wu)培(pei)(pei)養(yang)(yang)。目前,隨著科學水(shui)平的(de)(de)發展,培(pei)(pei)養(yang)(yang)箱(xiang)(xiang)(xiang)(xiang)設(she)備(bei)的(de)(de)完善程度(du)(du)和價(jia)格(ge)有(you)(you)很大(da)差別。有(you)(you)各(ge)種結構合理、功能(neng)齊全的(de)(de)培(pei)(pei)養(yang)(yang)箱(xiang)(xiang)(xiang)(xiang),如恒(heng)(heng)溫培(pei)(pei)養(yang)(yang)箱(xiang)(xiang)(xiang)(xiang)、恒(heng)(heng)溫恒(heng)(heng)濕培(pei)(pei)養(yang)(yang)箱(xiang)(xiang)(xiang)(xiang)、低溫培(pei)(pei)養(yang)(yang)箱(xiang)(xiang)(xiang)(xiang)、微(wei)(wei)生(sheng)物(wu)(wu)多(duo)用(yong)(yong)(yong)培(pei)(pei)養(yang)(yang)箱(xiang)(xiang)(xiang)(xiang)和二氧化碳培(pei)(pei)養(yang)(yang)箱(xiang)(xiang)(xiang)(xiang)等。有(you)(you)的(de)(de)用(yong)(yong)(yong)計算機控(kong)制,可(ke)選擇多(duo)條時(shi)間線(xian)變換溫差,從而克服了環(huan)境溫度(du)(du)的(de)(de)影(ying)響(xiang),一年(nian)四季均能(neng)達到(dao)培(pei)(pei)養(yang)(yang)要求的(de)(de)溫度(du)(du)。
微生物(wu)多用培(pei)養箱(xiang)是(shi)集(ji)加熱、制冷(leng)和(he)振蕩于一體(ti)的(de)微生物(wu)液體(ti)發酵裝(zhuang)置。工(gong)作室的(de)溫(wen)度(du)在(zai)15~50℃范圍內任(ren)意(yi)選(xuan)定(ding),選(xuan)定(ding)后經溫(wen)控(kong)儀自動控(kong)制,保(bao)持工(gong)作室內恒溫(wen)。同時設有可控(kong)硅調(diao)速系統,振蕩機(ji)轉速可在(zai)1~220rpm范圍內任(ren)意(yi)調(diao)控(kong)。
晾干箱干燥箱(xiang)是用于除(chu)去潮濕物(wu)料內(nei)及器皿(min)內(nei)外(wai)(wai)水(shui)分或其(qi)它揮發性溶液的設備(bei)。類型(xing)很多(duo),有箱(xiang)式(shi)、滾筒式(shi)、套(tao)間式(shi)、回轉式(shi)等。微(wei)生物(wu)學實驗室多(duo)用箱(xiang)式(shi)干燥箱(xiang),大小規格不一。工作室內(nei)配有可(ke)活動的鐵(tie)絲網板(ban),便于放置被(bei)干燥的物(wu)品。制(zhi)熱升溫(wen)式(shi)干燥箱(xiang)也是有電(dian)爐絲和溫(wen)度控(kong)制(zhi)儀組成,可(ke)調節(jie)溫(wen)度從室溫(wen)至300℃任意選擇。有的干燥箱(xiang)采用導電(dian)溫(wen)度計為敏感元件,配合(he)晶體管和繼電(dian)器組成自動控(kong)制(zhi)系統,克服了金(jin)屬管型(xing)熱膨(peng)脹控(kong)制(zhi)的缺點。此外(wai)(wai),還有真空干燥箱(xiang)(配有真空泵和氣壓(ya)(ya)表(biao)),可(ke)在常壓(ya)(ya)或減壓(ya)(ya)下操作。
搖床
搖床又稱搖瓶(ping)(ping)機(ji),它(ta)是(shi)培養好氣性微(wei)生(sheng)物(wu)的(de)小型試驗設備或(huo)(huo)作為種(zhong)子(zi)擴大(da)(da)培養之用,常用的(de)搖床有(you)往(wang)復(fu)(fu)式(shi)和旋轉式(shi)兩種(zhong)。往(wang)復(fu)(fu)式(shi)搖床的(de)往(wang)復(fu)(fu)頻(pin)率(lv)一(yi)(yi)般(ban)在80~140次/min,沖程一(yi)(yi)般(ban)為5~14cm,如頻(pin)率(lv)過快、沖程過大(da)(da)或(huo)(huo)瓶(ping)(ping)內液(ye)體裝(zhuang)量過多,在搖動時液(ye)體會濺到包扎瓶(ping)(ping)口的(de)紗布或(huo)(huo)棉塞上,導致雜菌污染,特別是(shi)啟動時更容易發生(sheng)這種(zhong)情(qing)況。旋轉式(shi)搖床的(de)偏心距(ju)一(yi)(yi)般(ban)在3~6cm之間,旋轉次數為60~300rpm。
放(fang)在搖床(chuang)上的(de)(de)培養瓶(一般為三角瓶)中的(de)(de)發酵液所需要的(de)(de)氧(yang)是由空氣(qi)經瓶口包扎的(de)(de)紗布(一般8層(ceng))或棉塞(sai)通入的(de)(de),所以氧(yang)的(de)(de)傳遞與瓶口的(de)(de)大小、瓶口的(de)(de)幾何形狀、棉塞(sai)或紗布的(de)(de)厚(hou)度和密度有(you)關。在通常情況下,搖瓶的(de)(de)氧(yang)吸收系數取(qu)決于(yu)搖床(chuang)的(de)(de)特性(xing)和三角瓶的(de)(de)裝(zhuang)樣量。
導桿式搖床是利用率曲柄原則推動搖床作導桿運行,主機為金屬制或實木的長方框子,有塊層至多層塑料托盤價格,塑料托盤價格上面園孔備放激發出瓶,孔中突出是一個角形形橡皮擦,用為確定激發出瓶并避免瓶的振功,齒輪變速箱貸款機構基本選取特殊三角帶輪低速,需要更改調壓三角帶輪可轉變導桿頻率。反力輪上都有區別的反力孔,能夠轉換反力距。導桿式搖床的頻率和反力距的各個對氧的消化吸收有看不出的作用。 旋轉式搖床是回收利用旋轉的軸力軸使包裝箱搖動,包裝箱有塊層或二層,用于304不銹鋼條、鋁合金板或木質板制作。在以下三個軸力軸里裝有聯接可變節上,使包裝箱保持著含量。那樣搖床框架更復雜,造貴點。其優點有哪些是氧的信息傳遞非常好、效率耗用小、培養基不容易濺到瓶口的毛巾上。 體視顯微鏡 微生態學每個人很小,務必推動光學顯微鏡了解這樣才能了解模糊這句話的每個人價值形式和神經元成分。為此,在微生態學學的各個設計中,光學顯微鏡了解就加入不能夠緊缺的專用工具。顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)的(de)種類很多,根據其(qi)結構,可以(yi)分(fen)為(wei)(wei)(wei)光(guang)(guang)學(xue)(xue)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)和(he)非(fei)光(guang)(guang)學(xue)(xue)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)兩大(da)類。光(guang)(guang)學(xue)(xue)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)又可分(fen)為(wei)(wei)(wei)單式(shi)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)和(he)復(fu)式(shi)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)。最(zui)簡單的(de)單式(shi)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)即放(fang)大(da)鏡(jing)(jing)(jing)(放(fang)大(da)倍數常在(zai)(zai)10倍左右),構造復(fu)雜(za)的(de)單式(shi)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)為(wei)(wei)(wei)解剖顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(放(fang)大(da)倍數在(zai)(zai)200左右)。在(zai)(zai)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)生物學(xue)(xue)的(de)研究中,主要是復(fu)式(shi)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)。其(qi)中以(yi)普(pu)通光(guang)(guang)學(xue)(xue)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(明視野顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing))最(zui)為(wei)(wei)(wei)常用。此外,還有暗視野顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)、相差顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)、熒光(guang)(guang)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)、偏光(guang)(guang)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)、紫外光(guang)(guang)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)和(he)倒置顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)等。非(fei)光(guang)(guang)學(xue)(xue)顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)為(wei)(wei)(wei)電子顯(xian)(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)。
打疫苗箱接(jie)種(zhong)箱(xiang)(xiang)分(fen)(fen)為固體(ti)(ti)菌(jun)種(zhong)接(jie)種(zhong)箱(xiang)(xiang)和(he)液體(ti)(ti)菌(jun)種(zhong)接(jie)種(zhong)箱(xiang)(xiang)兩(liang)(liang)種(zhong)。固體(ti)(ti)菌(jun)種(zhong)接(jie)種(zhong)箱(xiang)(xiang)是一個用木料和(he)玻(bo)(bo)(bo)(bo)璃(li)制(zhi)成或由有(you)機玻(bo)(bo)(bo)(bo)璃(li)焊接(jie)而(er)成的密(mi)閉(bi)小(xiao)箱(xiang)(xiang)。又分(fen)(fen)為雙(shuang)人和(he)單人操作箱(xiang)(xiang)。箱(xiang)(xiang)體(ti)(ti)可大可小(xiao),一般箱(xiang)(xiang)體(ti)(ti)長約(yue)143cm,寬(kuan)86cm,總高154cm,支架76cm。箱(xiang)(xiang)的上部(bu)左右兩(liang)(liang)側各裝(zhuang)有(you)兩(liang)(liang)扇能啟閉(bi)的玻(bo)(bo)(bo)(bo)璃(li)推拉門(men),方便菌(jun)種(zhong)進出(chu)。窗的下部(bu)分(fen)(fen)別設有(you)兩(liang)(liang)個直徑約(yue)13cm的圓洞(dong),兩(liang)(liang)洞(dong)的中心距離為52cm(同肩寬(kuan)),洞(dong)口裝(zhuang)有(you)帶松緊帶的袖套(tao),以防(fang)雙(shuang)手在(zai)箱(xiang)(xiang)內操作時(shi),外界空(kong)氣進入箱(xiang)(xiang)內造成污染。操作時(shi)兩(liang)(liang)人相對而(er)坐,雙(shuang)手通過袖套(tao)伸入箱(xiang)(xiang)內。箱(xiang)(xiang)兩(liang)(liang)側最好也(ye)裝(zhuang)上玻(bo)(bo)(bo)(bo)璃(li),箱(xiang)(xiang)頂部(bu)為木板(ban)或玻(bo)(bo)(bo)(bo)璃(li)。箱(xiang)(xiang)內頂部(bu)裝(zhuang)有(you)紫外線殺菌(jun)燈和(he)照(zhao)明用日光燈各一支。箱(xiang)(xiang)體(ti)(ti)安裝(zhuang)木板(ban)或玻(bo)(bo)(bo)(bo)璃(li)均可,但要注意密(mi)封。
液(ye)體(ti)菌(jun)種(zhong)(zhong)接種(zhong)(zhong)箱(xiang)(xiang)(xiang)是(shi)專為移(yi)接液(ye)體(ti)菌(jun)種(zhong)(zhong)而設(she)計的。比固體(ti)菌(jun)種(zhong)(zhong)箱(xiang)(xiang)(xiang)窄長(chang)(chang),單側兩人(ren)操(cao)作。內設(she)軌道和紫外線燈,箱(xiang)(xiang)(xiang)兩端開有高25cm,寬10cm的長(chang)(chang)方形出口(kou)(kou),方便菌(jun)種(zhong)(zhong)進出,洞(dong)口(kou)(kou)設(she)有小推門(men)。進出口(kou)(kou)下處設(she)蒸汽源(yuan),接種(zhong)(zhong)時用(yong)蒸汽封(feng)住進出口(kou)(kou),以防雜菌(jun)進入箱(xiang)(xiang)(xiang)內。箱(xiang)(xiang)(xiang)背面(mian)設(she)有液(ye)體(ti)菌(jun)種(zhong)(zhong)移(yi)接管(guan)能進入的小孔。
接(jie)種(zhong)(zhong)箱(xiang)滅菌(jun)時,用(yong)紫(zi)外線照射30min。如果沒(mei)有紫(zi)外線燈,可用(yong)甲(jia)醛和高(gao)錳酸(suan)鉀(jia)(甲(jia)醛10~14mL/m3+高(gao)錳酸(suan)鉀(jia)5~7g/m3空(kong)間)熏蒸(zheng)30min以上。使用(yong)時,先(xian)將所需(xu)物(wu)品(pin)和工具放入接(jie)種(zhong)(zhong)箱(xiang)內,然后進(jin)(jin)行(xing)藥劑熏蒸(zheng)和紫(zi)外線滅菌(jun),再按無菌(jun)操作(zuo)進(jin)(jin)行(xing)接(jie)種(zhong)(zhong)。接(jie)種(zhong)(zhong)箱(xiang)的結(jie)構簡單,造價低廉,易消毒滅菌(jun),操作(zuo)方便,而(er)且(qie)人在箱(xiang)外操作(zuo),氣溫較高(gao)時也能作(zuo)業(ye)。缺點是進(jin)(jin)出(chu)培養(yang)基費工費時,每次接(jie)種(zhong)(zhong)前都需(xu)要進(jin)(jin)行(xing)滅菌(jun)。
洗衣機 微生物實驗室的冰箱主要有兩種:普通冰箱和低溫冷凍冰箱。普通冰箱一般都具有兩個柜子,即鮮藏柜和冷藏柜,溫度分別為4℃和-20℃;低溫冷凍冰箱溫度一般控制在-40~-80℃。它們都可以用于微生物菌種保藏。鮮藏柜常用于保存斜面菌種,保藏時間在3個月左右。超過3個月,斜面就會變干,因此需要轉接菌種。如果要長時間保存菌種,則需要經過處理后,貯藏于普通冰箱的冷藏柜或低溫冷凍冰箱中,它們的保藏時間較長,一般都在1年以上。